不同培养条件对禽源沙门菌菌毛基因表达的影响

doi:10.11843/j.issn.0366-6964.2014.01.016

Abstract

Abstract:

The objective of the present study was to investigate the distribution of fimbrial genes in some avian Salmonella serotypes and the expression differences of fimbrial genes under different bacterial culture conditions.Seventeen kinds of fimbrial operon genes in Salmonella pullorum，Salmonella gallinarum，Salmonella typhimurium and Salmonella enteritidis were detected by PCR，and expression of fimbrial genes in the above Salmonella strains under three different culture conditions which include static broth，shaken broth and static plate were detected by
RT-PCR.Results showed that the four Salmonella serotypes had different fimbrial genotypes，10 of which were conservative，and the distribution of the other 7 fimbrial genes was different.RT-PCR detection results showed that the expression of fimbrial genes in different
serotypes cultured under three conditions had some differences，as the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis cultured on static broth was more than that of others (P<0.05)，while the difference in Salmonella gallinarum and Salmonella
typhimurium was not significant (P>0.05).The results revealed that the four Salmonella serotypes had different fimbrial genotypes and the bacterial culture condition significantly impacted the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis.

CLC Number:

S852.612

GONG Jian-sen，WANG Xin，HAN Xian-gan，ZHU Chun-hong，YU Yan，LIU Xue-xian，XU Bu. Effects of Different Bacterial Culture Condition on the Expression of Avian Salmonella Fimbrial Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 116-122.

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References

［1］HERIKSTAD H，MOTARJEMI Y，TAUXE R V.Salmonella surveillance：a global survey of public health serotyping［J］.Epidemiol Infect，2002，129(1)：1-8.

［2］MAJOWICZ S E，MUSTO J，SCALLAN E，et al.The global burden of nontyphoidal Salmonella gastroenteritis［J］.Clin Infect Dis，2010，50(6)：882-889.

［3］ABBOTT S L，NI F C，JANDA J M.Increase in extraintestinal infections caused by Salmonella enterica subspecies II–IV［J］.Emerg Infect Dis，2012，18(4):637-639.

［4］FOLEY S L，NAYAK R，HANNING I B，et al.Population dynamics of Salmonella enterica serotypes in commercial egg and poultry production［J］.Appl Environ Microbiol，2011，77(13)：4273-4279.

［5］LIMAWONGPRANEE S，HAYASHIDANI H，OKATANI A T，et al.Prevalence and persistence of Salmonella in broiler chicken flocks［J］.J Vet Med Sci，1999，61(3)：255-259.

［6］SANCHEZ S，HOFACRE C L，LEE M D，et al.Animal sources of salmonellosis in humans［J］.J Am Vet Med Assoc，2002，221(4)：492-497.

［7］张燕，朱超.我国沙门氏菌病和菌型分布概况［J］.现代预防医学，2002，29(3)：400-401.

［8］BARROW P A，FREITAS NETO O C.Pullorum disease and fowl typhoid-new thoughts on old diseases：a review［J］.Avian Pathol，2011，40(1)：1-13.

［9］BARROW P A，JONES M A，SMITH A L，et al.The long view：Salmonella—the last forty years［J］.Avian Pathol，2012，41(5)：413-420.

［10］况彗星，韦婷.禽沙门氏菌的检测与防制［J］.中国家禽，2003，25(4)：4-7.

［11］徐仕忠.家禽沙门氏菌病及其疫苗控制［J］.中国家禽，2006，28(17)：48-50.

［12］RAJASHEKARA G，MUNIR S，ALEXEYEV M F，et al.Pathogenic role of SEF14，SEF17，and SEF21 fimbriae in Salmonella enterica serovar enteritidis infection of chickens［J］.Appl Environ Microbiol，2000，66(4)：1759-1763.

［13］ASLANZADEH J，PAULISSEN L J.Adherence and pathogenesis of Salmonella enteritidis in mice［J］.Microbiol Immunol，1990，34(11)：885-893.

［14］MIRMOMENI M H，SISAKHTNEZHAD S，SHARIFI A.Rapid detection of Salmonella enteritidis by PCR amplification of the SefA gene and it’s cloning［J］.Pak J Biol Sci，2008，11(3)：428-432.

［15］朱春红，吴娟，张伟娟，等.肠炎沙门氏菌SEFA基因表达和间接ELISA检测方法的初步建立［J］.中国预防兽医学报，2010，32(1)：44-48.

［16］朱春红，孟霞，厚华艳，等.沙门氏菌菌毛研究进展［J］.中国动物传染病学报，2013，21(2)：68-74.

［17］ZHU C，MENG X，DUAN X，et al.SEF14 fimbriae from Salmonella enteritidis play a role in pathogenitic to cell model in vitro and host in vivo［J］.Microb Pathog，2013，64C：18-22.

［18］MORGAN E，CAMPBELL J D，ROWE S C，et al.Identification of host-specific colonisation factors of Salmonella enterica serovar Typhimurium［J］.Mol Microbiol，2004，54(4)：994-1010.

［19］LEDEBOER N A，FRYE J G，MCCLELLAND M，et al.Salmonella enterica serovar Typhimurium requires the Lpf，Pef，and Tafi fimbriae for biofilm formation on HEp-2 tissue culture cells and chicken intestinal epithelium［J］.Infect Immun，2006，74(6)：3156-3169.

［20］BUMLER A J，TSOLIS R M，BOWE F A，et al.The pef operon of Salmonella typhimurium mediates adhesion to murine small intestine and is necessary for fluid accumulation in the infant mouse［J］.Infect Immun，1996，64(1)：61-68.

［21］BUMLER A J，TSOLIS R M，HEFFRON F.The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer’s patches［J］.Proc Natl Acad Sci U S A，1996，93(1)：279-283.

［22］ZHU C H，HASSAN H M，WU S L，et al.The role of SEF14 fimbriae in pathogenesis and enhancing the immunity of Salmonella enteritidis［J］.Afr J Microbiol Res，2009，3(5)：191-194.

［23］SELANDER R K，BELTRAN P，SMITH N H，et al.Evolutionary genetic relationships of clones of Salmonella serovars that cause human typhoid and other enteric fevers ［J］. Infect Immun，1990，58(7)：2262-2275.

［24］NORRIS T L，BUMLER A J.Phase variation of the lpf operon is a mechanism to evade cross-immunity between Salmonella serotypes ［J］.Proc Natl Acad Sci U S A，1999，96(23)：13393-13398.

［25］KINGSLEY R A，BUMLER A J.Host adaptation and the emergence of infectious disease：the Salmonella paradigm［J］.Mol Microbiol，2000，36(5)：1006-1014.

［26］OCHOA-REPÁRAZ J，SESMA B，ALVAREZ M，et al.Humoral immune response in hens naturally infected with Salmonella enteritidis against outer membrane proteins and other
surface structural antigens［J］.Vet Res，2004，35(3)：291-298.

［27］WALKER S L，SOJKA M，DIBB-FULLER M，et al.Effect of pH，temperature and surface contact on the elaboration of fimbriae and
flagella by Salmonella serotype Enteritidis［J］. J Med Microbiol，1999，48 (3)：253-261.

［28］CHUANG Y C，WANG K C，CHEN Y T，et al.Identification of the genetic determinants of Salmonella enterica serotype Typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agal and static broth culture［J］.BMC Microbiol，2008，25(8)：126-137.

［29］YOO A Y，YU J E，YOO H，et al.Role of sigma factor E in regulation of Salmonella Agf expression［J］.Biochem Biophys Res Commun，2013，430(1)：131-136.

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Effects of Different Bacterial Culture Condition on the Expression of Avian Salmonella Fimbrial Genes

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